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primescript tm rt mastermix kit  (TaKaRa)


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    Structured Review

    TaKaRa primescript tm rt mastermix kit
    Primescript Tm Rt Mastermix Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 26665 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescript+tm+rt+mastermix+kit/PrimeScript+RT+Master+Mix/pm40383604-129-43-48
    Average 99 stars, based on 26665 article reviews
    primescript tm rt mastermix kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Rocaglamide enhances NK cell-mediated killing of non-small cell lung cancer cells by inhibiting autophagy
    Article Snippet: .. Quantitative real-time PCR A total of 3 × 10 5 of H460 or H1975 cells were seeded in 6-cm plates and incubated with different concentrations (0, 125, 250 and 500 nM) of RocA for 24 h. Total RNA was extracted using the MiniBEST Universal RNA extraction kit (TaKaRa, 9767), and 1 μg of total RNA was reverse transcribed into cDNA using the PrimeScript TM RT MasterMix kit (TaKaRa, RR036A) according to the manufacturer’s instructions. .. Real-time PCR was performed using a SYBR® Premix Ex TaqTM kit (TaKaRa, RR420A) according to the manufacturer’s instructions on an ABI system (Applied Biosystems, Thermo Fisher Scientific Inc., MA, USA).

    Incubation:

    Article Title: Rocaglamide enhances NK cell-mediated killing of non-small cell lung cancer cells by inhibiting autophagy
    Article Snippet: .. Quantitative real-time PCR A total of 3 × 10 5 of H460 or H1975 cells were seeded in 6-cm plates and incubated with different concentrations (0, 125, 250 and 500 nM) of RocA for 24 h. Total RNA was extracted using the MiniBEST Universal RNA extraction kit (TaKaRa, 9767), and 1 μg of total RNA was reverse transcribed into cDNA using the PrimeScript TM RT MasterMix kit (TaKaRa, RR036A) according to the manufacturer’s instructions. .. Real-time PCR was performed using a SYBR® Premix Ex TaqTM kit (TaKaRa, RR420A) according to the manufacturer’s instructions on an ABI system (Applied Biosystems, Thermo Fisher Scientific Inc., MA, USA).

    Article Title: Rocaglamide enhances NK cell-mediated killing of non-small cell lung cancer cells by inhibiting autophagy
    Article Snippet: .. A total of 3 × 10 5 of H460 or H1975 cells were seeded in 6-cm plates and incubated with different concentrations (0, 125, 250 and 500 nM) of RocA for 24 h. Total RNA was extracted using the MiniBEST Universal RNA extraction kit (TaKaRa, 9767), and 1 μg of total RNA was reverse transcribed into cDNA using the PrimeScript TM RT MasterMix kit (TaKaRa, RR036A) according to the manufacturer’s instructions. .. Real-time PCR was performed using a SYBR® Premix Ex TaqTM kit (TaKaRa, RR420A) according to the manufacturer’s instructions on an ABI system (Applied Biosystems, Thermo Fisher Scientific Inc., MA, USA).

    RNA Extraction:

    Article Title: Rocaglamide enhances NK cell-mediated killing of non-small cell lung cancer cells by inhibiting autophagy
    Article Snippet: .. Quantitative real-time PCR A total of 3 × 10 5 of H460 or H1975 cells were seeded in 6-cm plates and incubated with different concentrations (0, 125, 250 and 500 nM) of RocA for 24 h. Total RNA was extracted using the MiniBEST Universal RNA extraction kit (TaKaRa, 9767), and 1 μg of total RNA was reverse transcribed into cDNA using the PrimeScript TM RT MasterMix kit (TaKaRa, RR036A) according to the manufacturer’s instructions. .. Real-time PCR was performed using a SYBR® Premix Ex TaqTM kit (TaKaRa, RR420A) according to the manufacturer’s instructions on an ABI system (Applied Biosystems, Thermo Fisher Scientific Inc., MA, USA).

    Article Title: Rocaglamide enhances NK cell-mediated killing of non-small cell lung cancer cells by inhibiting autophagy
    Article Snippet: .. A total of 3 × 10 5 of H460 or H1975 cells were seeded in 6-cm plates and incubated with different concentrations (0, 125, 250 and 500 nM) of RocA for 24 h. Total RNA was extracted using the MiniBEST Universal RNA extraction kit (TaKaRa, 9767), and 1 μg of total RNA was reverse transcribed into cDNA using the PrimeScript TM RT MasterMix kit (TaKaRa, RR036A) according to the manufacturer’s instructions. .. Real-time PCR was performed using a SYBR® Premix Ex TaqTM kit (TaKaRa, RR420A) according to the manufacturer’s instructions on an ABI system (Applied Biosystems, Thermo Fisher Scientific Inc., MA, USA).

    Reverse Transcription:

    Article Title: Rocaglamide enhances NK cell-mediated killing of non-small cell lung cancer cells by inhibiting autophagy
    Article Snippet: .. Quantitative real-time PCR A total of 3 × 10 5 of H460 or H1975 cells were seeded in 6-cm plates and incubated with different concentrations (0, 125, 250 and 500 nM) of RocA for 24 h. Total RNA was extracted using the MiniBEST Universal RNA extraction kit (TaKaRa, 9767), and 1 μg of total RNA was reverse transcribed into cDNA using the PrimeScript TM RT MasterMix kit (TaKaRa, RR036A) according to the manufacturer’s instructions. .. Real-time PCR was performed using a SYBR® Premix Ex TaqTM kit (TaKaRa, RR420A) according to the manufacturer’s instructions on an ABI system (Applied Biosystems, Thermo Fisher Scientific Inc., MA, USA).

    Article Title: MiR-371b-5p reduces osteosarcoma cell migration and proliferation to induce apoptosis by targeting FUT4
    Article Snippet: The quantity of RNA was determined using a UV spectrophotometer (Hitachi, Tokyo, Japan). .. The extracted RNA was then reverse-transcribed into complementary deoxyribonucleic acid (cDNA) by the PrimeScript TM RT MasterMix kit (RR036A, Takara Bio Inc., Beijing, China). .. SYBR Green Master Mix (A46012, Applied Biosystems, Foster, CA, USA) was carried out in the quantitative real-time polymerase chain reaction (qRT-PCR) assay.

    Article Title: Rocaglamide enhances NK cell-mediated killing of non-small cell lung cancer cells by inhibiting autophagy
    Article Snippet: .. A total of 3 × 10 5 of H460 or H1975 cells were seeded in 6-cm plates and incubated with different concentrations (0, 125, 250 and 500 nM) of RocA for 24 h. Total RNA was extracted using the MiniBEST Universal RNA extraction kit (TaKaRa, 9767), and 1 μg of total RNA was reverse transcribed into cDNA using the PrimeScript TM RT MasterMix kit (TaKaRa, RR036A) according to the manufacturer’s instructions. .. Real-time PCR was performed using a SYBR® Premix Ex TaqTM kit (TaKaRa, RR420A) according to the manufacturer’s instructions on an ABI system (Applied Biosystems, Thermo Fisher Scientific Inc., MA, USA).

    Article Title: Circular RNA hsa_circ_0049657 as a Potential Biomarker in Non-Small Cell Lung Cancer
    Article Snippet: RNA concentration was measured using an ultraviolet spectrophotometer (IMPLEN, Germany). .. Subsequently, extracted RNA was reverse transcribed into cDNA according to the instructions of the PrimeScript TM RT MasterMix kit (Takara, Dalian, China). .. PCRs were performed by using the SYBR Green method in an ABI 7500 FAST sequence detection system (Applied Biosystems).

    Synthesized:

    Article Title: A Novel NAC Transcription Factor From Eucalyptus , EgNAC141, Positively Regulates Lignin Biosynthesis and Increases Lignin Deposition
    Article Snippet: Genomic DNA was removed by DNase I (Takara) treatment. .. First-strand cDNA was synthesized using a PrimeScript TM RT MasterMix kit (Takara). .. Real-time quantitative PCR (RT-qPCR) was performed using Novostar-SYBR Supermix (Novoprotein, Shanghai, China) with the Thermal Cycler Dice Real Time System TP950 (TaKaRa, Japan).

    Extraction:

    Article Title: Structural characterization and immune-modulating properties of a novel β-galactomannan extracted from black soybean hulls.
    Article Snippet: Galactomannan soluble in water (BBHW) was extracted from black soybean hulls through acid-assisted extraction and column chromatography and its immune-modulating capacity and features of the structure were elucidated.. BBHW had an average molecular mass of 134,194 Da and was made up of mannose, galactose, glucose, and arabinose in a molar ratio of 1.40:1.0:0.14:0.03.. The backbone structure of BBHW were primarily composed of →4)-β-D-Manp-(1 → and →4,6)-β-D-Manp-(1→, branched with α-D-Galp-(1→, α-D-Galp-(1 → 6)-α-DGlcp-(1→, and α-L-Araf-(1 → at O-6.

    cDNA Synthesis:

    Article Title: Structural characterization and immune-modulating properties of a novel β-galactomannan extracted from black soybean hulls.
    Article Snippet: Galactomannan soluble in water (BBHW) was extracted from black soybean hulls through acid-assisted extraction and column chromatography and its immune-modulating capacity and features of the structure were elucidated.. BBHW had an average molecular mass of 134,194 Da and was made up of mannose, galactose, glucose, and arabinose in a molar ratio of 1.40:1.0:0.14:0.03.. The backbone structure of BBHW were primarily composed of →4)-β-D-Manp-(1 → and →4,6)-β-D-Manp-(1→, branched with α-D-Galp-(1→, α-D-Galp-(1 → 6)-α-DGlcp-(1→, and α-L-Araf-(1 → at O-6.



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